Effect of fresh soy milk and its compounds on apoptosis in human leukemic cells and peripheral blood mononuclear cells


 Purpose: To determine the effect of fresh soy milk and its compounds (coumestrol, daidzein genistein) on apoptosis in human leukemic cells peripheral blood mononuclear (PBMC).
 Methods: The apoptotic (K562, Jurkat, U937) PBMC was determined by flow cytometry. from healthy donors were isolated conducting density gradient centrifugation principle. Lymphoprep cytotoxicity evaluated 3-(4,5- ‘;1dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) MTT) assay.
 Results: treatment genistein had a significantly higher half-maximal inhibitory concentration (IC50) value (p < 0.01) 0.0001), compared to leukemia cells. In addition, IC50 0.05) than Coumestrol induced increased K562, U937 Jurkat 0.05). However, only K562 U937. Genistein reported cell 0.001). Nonetheless, PBMC, significant did not occur. 0.05).
 Conclusion: findings indicate that coumestrol, daidzein, inhibits viability induction, but they are non-toxic toward normal Therefore, results suggest compound alone, thus, might have therapeutic leukemia.